| New Smyrna Beach Brewing Co | 143 Canal St | New Smyrna Beach | Florida | 32168-7067 | United States | micro | |
| NightLife Brewing Co | 1588 Nw 7th St | Miami | Florida | 33125 | United States | micro | |
| Nivol Brewing | 483 N Richard Jackson Blvd | Panama City Beach | Florida | 32407-3647 | United States | micro | |
| NOBO Brewing Company | 2901 Commerce Park Dr | Boynton Beach | Florida | 33426-8728 | United States | micro | |
| Ocean Sun Brewing | 3030 Curry Ford Road | Orlando | Florida | 32806-3376 | United States | micro | |
| Odd Breed Wild Ales | 50 NE 1st St | Pompano Beach | Florida | 33060-6602 | United States | micro | |
| Old Coast Ales | 300 Anastasia Blvd Ste C | Saint Augustine | Florida | 32080-4759 | United States | micro | |
| Old Soul Brewing | 10970 S Cleveland Ave Ste 402 | Fort Myers | Florida | 33907-2315 | United States | micro | |
| Ology Brewing Co | 118 E 6th Ave | Tallahassee | Florida | 32303 | United States | micro | |
| Ookapow Brewing Company | 1142 Old Okeechobee Road, Bay 13 | West Palm Beach | Florida | 33401 | United States | micro | |
| Orange Blossom Brewing Company | 44 W Illiana St Ste 102 | Orlando | Florida | 32806-4439 | United States | proprietor | |
| Orange County Brewers | 131 N Orange Ave Ste 106 | Orlando | Florida | 32801-2385 | United States | brewpub | |
| Orchid Island Brewery | 2855 Ocean Dr Ste C1 | Vero Beach | Florida | 32963-2038 | United States | micro | |
| Orlando Brewing Partners, Inc. | 1301 Atlanta Ave | Orlando | Florida | 32806-3908 | United States | micro | |
| Ormond Brewing | 301 Division Ave Unit 15 | Ormond Beach | Florida | 32174-8812 | United States | micro | |
| Overflow Brewing Company | 770 1st Ave N | Saint Petersburg | Florida | 33701 | United States | micro | |
| Oviedo Brewing Co | | Oviedo | Florida | 32765-7426 | United States | planning | |
| Oyster City Brewing Company | 17 Avenue D | Apalachicola | Florida | 32320-1801 | United States | micro | |
| Pair O' Dice Brewing Company | 4400 118th Ave N Ste 208 | Clearwater | Florida | 33762-4433 | United States | micro | |
| Palm City Brewing | 7887 Drew Cir Ste 130 | Fort Myers | Florida | 33967-6083 | United States | micro | |
| Palm Harbor Brewery | 1022 Georgia Ave | Palm Harbor | Florida | 34683-4317 | United States | brewpub | |
| Pareidolia Brewing Co. | 712 Cleveland St | Sebastian | Florida | 32958-4178 | United States | brewpub | |
| Pensacola Bay Brewery | 225 E Zaragoza St | Pensacola | Florida | 32502-6048 | United States | micro | |
| Perfect Plain Brewing Co. | 50 E Garden St | Pensacola | Florida | 32502-5604 | United States | micro | |
| Persimmon Hollow Brewing | 111 W Georgia Ave | Deland | Florida | 32720-5406 | United States | micro | |
| Pesky Pelican Brewpub | 923 72nd St N | Saint Petersburg | Florida | 33710-4666 | United States | brewpub | |
| Pinellas Ale Works | 1962 1st Ave S | Saint Petersburg | Florida | 33712-1345 | United States | micro | |
| Pinglehead Brewery / Brewer's Pizza | 14B Blanding Blvd Ste B | Orange Park | Florida | 32073-2232 | United States | brewpub | |
| Playalinda Brewing Company | 305 S Washington Ave | Titusville | Florida | 32796-3539 | United States | micro | |
| Playalinda Brewing Company - Brix Project | 5220 S Washington Ave | Titusville | Florida | 32780-7316 | United States | brewpub | |
| Point Ybel Brewing Company | 16120 San Carlos Blvd Ste 4 | Fort Myers | Florida | 33908-3294 | United States | micro | |
| Proof Brewing Co | 644 McDonnell Dr | Tallahassee | Florida | 32310-4808 | United States | micro | |
| Props Brewery and Grill | 125 Lovejoy Rd | Fort Walton Beach | Florida | 32548 | United States | brewpub | |
| Props Craft Brewery Taproom | 125 Lovejoy Rd NW | Fort Walton Beach | Florida | 32548-3418 | United States | micro | |
| Ragtime Tavern Seafood and Grill | 207 Atlantic Blvd | Atlantic Beach | Florida | 32233-5273 | United States | brewpub | |
| Rapp Brewing Company | 10930 Endeavour Way Ste C | Seminole | Florida | 33777-1632 | United States | micro | |
| Red Cypress Brewery | 855 E SR 434 | Winter Springs | Florida | 32708-2704 | United States | micro | |
| Redlight Redlight Beer Parlour and Brewery | 2810 Corrine Dr | Orlando | Florida | 32803-2226 | United States | micro | |
| Right Around The Corner | 2244 Central Ave | Saint Petersburg | Florida | 33712 | United States | micro | |
| Riptide Brewing Company | 987 3rd Ave N | Naples | Florida | 34102-5810 | United States | micro | |
| River City Brewing Co | 835 Museum Cir | Jacksonville | Florida | 32207-9003 | United States | brewpub | |
| Rock Bottom Brewery | 1864 Victory Cir Bldg K | Daytona Beach | Florida | 32114 | United States | brewpub | |
| RockPit Brewing | 10 West Illiana St | Orlando | Florida | 32806 | United States | micro | |
| RoosterBragh Brewing Company, LLC | | Tallahassee | Florida | 32312-8000 | United States | planning | |
| Royal Palm Brewing Company | 543 N State Road 7 Ste 103 | Royal Palm Beach | Florida | 33411-3516 | United States | brewpub | |
| Sailfish Brewing Company, llc | 130 N 2nd St | Fort Pierce | Florida | 34950-4403 | United States | micro | |
| Saint Somewhere Brewing Co | 312 E Tarpon Ave | Tarpon Springs | Florida | 34689-4320 | United States | micro | |
| Saltwater Brewery | 1701 W Atlantic Ave | Delray Beach | Florida | 33444-1566 | United States | micro | |
| Sanford Brewing Company | 400 Sanford Ave | Sanford | Florida | 32771-1971 | United States | brewpub | |
| Sarasota Brewing Co | 6607 Gateway Ave | Sarasota | Florida | 34231-5805 | United States | brewpub | |